Shrimp Tropomyosin Detection

This is a straightforward and highly sensitive immunosensor that is developed by immobilising oxidised carbon nanohorns/Nafion/Fe3O4@Pd nanocomposite on carbon screen-printed electrodes (SPEs) for the detection of shrimp tropomyosin (Tro-Ag). The efficacy of the fabricated immunosensor was investigated via electrochemiluminescence (ECL) method, resulting from the chemical reaction between tris(bipyridine)ruthenium(II) chloride ([Ru(bpy)3]Cl2) and tripropylamine (TPrA). The nanocomposite was able to enhance the ECL intensity of [Ru(bpy)3]2+/TPrA system and successfully achieved exceptional sensitivity of 1 fg/mL (≈ 0.001 pg/mL) with a dynamic working range of 100 ng/mL to 1 fg/mL. Furthermore, this immunosensor displayed the capacity to be highly specific for Tro-Ag detection in food products with distinguished repeatability.