Meats are very prone to adulterations and mislabeling and despite proper labelling, doubts are inevitable due to the past cases of fraudulent labelling by dishonest manufacturers. The invention discloses a qPCR assay using specific primer and ZEN probe to detect porcine adulteration in food. The assay is highly specific to porcine DNA from nine other species. Porcine DNA was successfully detected in real food samples. The limit of detection in standard sample was as low as 1pg/μl and in raw pork‐chicken was as low as 0.001%. The assay rapidly detected 10ng/μl of porcine DNA approximately in 10.70 minutes.