U85: Electrochemiluminescence CD63 nanoimmunosensor using a carbon nanochips/iron oxide/nafion-nanocomposite

The detection of extracellular vesicles, or exosomes are important mediators in intercellular communication and often play a role in cancer progression. CD63 is a key exosomal protein due to its distinctive cellular functions and association with many cancers. This proposed study describes a a unique-highly sensitive, label-free electrochemiluminescence (ECL) nanoimmunosensor for the detection of CD63 protein over mesoporous carbon screen-printed electrode (MC-SPE) modified with novel nanocomposite of carbon nanochips (CNCs), iron oxide (Fe₃O₄) and nafion (NAF). Fourier-transform infrared spectroscopy (FTIR) and Field emission scanning electron microscopy (FE-SEM) were used to analyse nanocomposite. All the analytical performance of fabricated CD63 immunosensor were conducted applying ECL. In spite of the simple fabrication strategies utilized, the fabricated immunosensor showcased a broad linear range to detect CD63 from 100 fg mL^-1 to 10 ng mL^-1, with a limit of detection of 100 fg mL^-1, excellent selectivity, interference-resistance capability and potential to detect CD63 in real clinical samples.